Screen Quest 活細(xì)胞cAMP檢測（含細(xì)胞系）

G蛋白偶聯(lián)受體（GPCR）是藥品發(fā)現(xiàn)計(jì)劃中大的靶向受體之一。鈣通量（通過Gq途徑耦合）分析是篩選GPCR靶點(diǎn)的方法。然而，超過60%的已知GPCRs信號(hào)通過腺苷酸環(huán)化酶活性偶聯(lián)到cAMP。現(xiàn)有的cAMP檢測方法不僅需要細(xì)胞裂解，而且缺乏時(shí)間和空間分辨率。Screen Quest 活細(xì)胞cAMP檢測以高通量的形式提供細(xì)胞內(nèi)cAMP變化的實(shí)時(shí)監(jiān)測，無需細(xì)胞裂解步驟。該方法通過含有外源性環(huán)核苷酸門控通道（CNGC）或混雜G蛋白Gα16的細(xì)胞系進(jìn)行。該通道被細(xì)胞內(nèi)cAMP水平升高，導(dǎo)致離子通量和細(xì)胞膜去極化，可通過熒光鈣（如Calbryte 520 AM、Cal-520 AM、Fluo-8 AM或Fluo-4 AM和相應(yīng)的免洗鈣試劑盒）或熒光膜電位染料檢測到。Gα16與特異性非Gq偶聯(lián)受體的共表達(dá)將在受體刺激下產(chǎn)生細(xì)胞內(nèi)鈣信號(hào)。Screen Quest 活細(xì)胞cAMP檢測提供細(xì)胞系和試劑，用于使用FLIPR、FDSS或其他等效的熒光酶標(biāo)儀檢測細(xì)胞內(nèi)cAMP的變化。它已成功地用于測定Gs和Gi偶聯(lián)GPCR活性。百螢生物是AAT Bioquest的中國代理商，為您提供的Screen Quest 活細(xì)胞cAMP檢測。 

參考文獻(xiàn)

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
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Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling
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Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism
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Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons
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Journal: J Exp Biol (2016): 1798

Role of the cAMP Pathway in Glucose and Lipid Metabolism
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Journal: Handb Exp Pharmacol (2016): 29