Helixyte Green dsDNA染料用于PCR產(chǎn)物的直接循環(huán)測(cè)序方法中的PCR擴(kuò)增產(chǎn)量
Ex (nm) | 502 | Em (nm) | 522 |
分子量 | 661.17 | 溶劑 | DMSO |
存儲(chǔ)條件 | 在零下15度以下保存, 避免光照 |
Helixyte Green dsDNA染料是美國AAT Bioquest生產(chǎn)的一種超靈敏熒光核酸染料,用于定量溶液中的雙鏈DNA(dsDNA)。近期,Helixyte Green dsDNA染料用于PCR產(chǎn)物的直接循環(huán)測(cè)序方法中的PCR擴(kuò)增產(chǎn)量。使用標(biāo)準(zhǔn)光譜熒光計(jì)和2.5 ng / mL帶有熒光酶標(biāo)儀的dsDNA檢測(cè)到少至25 pg / mL的dsDNA(在2 mL測(cè)定體積中50 pg dsDNA),在ssDNA,RNA和ss存在下檢測(cè)效果小 游離核苷酸。該測(cè)定在三個(gè)數(shù)量級(jí)上是線性的并且?guī)缀鯖]有序列依賴性。它是從多種來源準(zhǔn)確測(cè)量DNA的理想選擇,包括基因組DNA,病毒DNA,小量制備DNA或PCR。百螢生物是AAT Bioquest的中國代理商,為您提供的Helixyte Green 雙鏈DNA定量試劑
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Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay
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Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding
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