基本信息
貨號(hào)：12500 (1mg/1mL)
儲(chǔ)存方式：保持在-20 oC。到期日為自收到之日起一年。
CAS號(hào)：61970-00-1
分子量：~60kDa
形式：液體（1mg / mL）
測(cè)量范圍：≤1飛摩爾ATP敏感度（使用0.2μg熒光素酶和適當(dāng)敏感的液體閃爍計(jì)數(shù)器或發(fā)光計(jì)）
生物應(yīng)用
該重組螢火蟲熒光素酶是從來(lái)自北美螢火蟲（Photinus pyralis）的克隆基因表達(dá)的酶，其提供使用生物發(fā)光試劑檢測(cè)ATP或熒光素底物進(jìn)行研究或生產(chǎn)試劑盒所需的可靠性和可靠性。 該重組酶可以潛在地消除可能在從天然來(lái)源純化的熒光素酶中發(fā)現(xiàn)的季節(jié)性和區(qū)域變異性的可能性。
該酶與熒光素，ATP和O2的反應(yīng)導(dǎo)致光的發(fā)射。 熒光素酶可用于檢測(cè)痕量的ATP。 螢火蟲熒光素酶也是基因表達(dá)研究中*常用的報(bào)告基因之一。 熒光素酶催化的生物發(fā)光反應(yīng)是測(cè)量基因表達(dá)的*靈敏的分析工具之一。 通過(guò)使用0.2μg熒光素酶可以檢測(cè)小于或等于1飛摩爾的ATP。
參考文獻(xiàn)
1. McElroy, W.D. (1947) The Energy Source for Bioluminescence in an isolated System. Proc. Natl. Acad. Sci. USA 33,342.
2. de Wet JR, Wood KV, Helinski DR, DeLuca M, (1985) Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli, Proc. Natl. Acad. Sci USA 82,7870-7873.
3. Khan, H.A. (2003) Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity, J. Bioscience 28, 379-382.
4. Drew, B and C. Leeuwenburgh (2003) Method for measuring ATP production in isolated mitochondria: ATP production in brain and liver mitochondria fo Fischer-344 rats with age and caloric restriction, Am J. Physiol. Regul. Integr. Comp. Physiol., 285, R1260-R1268.
5. Hara, K. Y. and Mori, H. (2006) An efficient method for quantitative determination of cellular ATP synthetic activity, J Biomol Screen 11, 310-7.
6. Sun, Y. and Chai, T. C. (2006) Augmented extracellular ATP signaling in bladder urothelial cells from patients with interstitial cystitis Am J Physiol Cell Physiol 290, C27-34.
7. Stanley, P.E., A review of bioluminescent ATP techniques in rapid microbiology. J. Biolumin. Chemilumin. 4, 375, (1989)
8. Rajgopal, S., and Vijayalakshmi, M.A. Enzyme Microb. Technol. 6, 482-489, (1984)
9. Chappelle, E.W., et al., Determination of bacterial content in fluids,. Meth. Enzymol. 57, 65-72, (1978)
10. Kricka, L.J., Clinical and biochemical applications of luciferases and luciferins. Anal. Biochem. 175, 14-21, (1988)
11. Liu L, Hastings JW. (2006) Two different domains of the luciferase gene in theheterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species. Proc Natl Acad Sci U S A. 12. Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M. (2006) cDNA cloning, expression and homology modeling of a luciferase from the firefly Lampyroidea maculata. J Biochem Mol Biol, 39, 578.
12. Viviani VR, Ohmiya Y. (2006) Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours. Luminescence, 21, 262.
13. Schipper ML, Patel MR, Gambhir SS. (2006) Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells. Mol Imaging Biol, 8, 218.
14. Oba Y, Sato M, Inouye S. (2006) Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor. Insect Mol Biol, 15, 293.
15. Palomba S, Berovic N, Palmer RE. (2006) Bioluminescence of monolayers of firefly luciferase immobilized on graphite. Langmuir, 22, 5451.
16. Schipper ML, Patel MR, Gambhir SS. (2006) Evaluation of Firefly Luciferase Bioluminescence Mediated Photodynamic Toxicity in Cancer Cells. Mol Imaging Biol.
18. Fraga H, Fernandes D, Novotny J, Fontes R, Esteves da Silva JC. (2006) Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation. Chembiochem, 7, 929.